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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Hypoxia promotes differentiation of adipose-derived stem cells into endothelial cells through demethylation of ephrinB2

Fig. 5

Differentiation of hASCs into vascular ECs is modulated through demethylation of ephrinB2 with exposure to hypoxia. Expression of CD31, Flk-1, and VE-cadherin under hypoxia condition was determined by a real-time PCR and b immunofluorescent staining. Scale bar: 50 um. c Real-time PCR analysis shows that hypoxia alone enhances expression of ephrinB2 in hASCs in a time-dependent manner. d Real-time PCR data showing that ephrinB2 expression was significantly increased in hASCs treated with VEGF and BMP4 in combination under hypoxia. e Expression of CD31, flk-1, and VE-cadherin was significantly decreased with addition of neutralizing agent against ephreinB2 as determined by real-time PCR. f Expression of ephrinB2 was detected by real-time PCR at 72 h after addition of DAC (1.0 uM, 2.5 uM), respectively. Data are presented as mean ± SD. *P < 0.05

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