Fig. 1

Characterization of induced pluripotent stem cells (iPSCs) derived from urinary cells. a iPSCs exhibiting ESC-like morphology in coculture with mouse embryonic feeder fibroblasts or in culture with mTeSR1. Scale bar, 100 μm. b Alkaline phosphatase staining of iPSCs. Scale bar, 100 μm. c Immunofluorescence staining for expression of OCT4, NANOG, SSEA4, TRA-1-81, TRA-1-60, and SSEA4 in iPSCs. Nuclei were counterstained with 4′, 6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 200 μm. d PCR assays for expression of OCT4 (endo), SOX2 (endo), KLF4 (endo), and c-Myc (endo) in iPSCs and parental urinary cells. e H&E staining of teratomas from NOD-SCID mice showing gut epithelium in the endoderm, neural epithelium in the ectoderm, and cartilage in the mesoderm. Scale bar, 100 μm