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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Feasibility of repairing full-thickness skin defects by iPSC-derived epithelial stem cells seeded on a human acellular amniotic membrane

Fig. 3

Properties of the hAAM and iPSC-derived EpSCs cultured on a hAAM. a H&E staining showing that epithelial cells were removed from the hAAM. Scale bar, 200 μm. b Three-dimensional porous morphology of hAAM detected by scanning electron microscopy. Scale bar, 100 μm. c, d iPSC-derived EpSCs cultured on a hAAM were observed at day 4 after loading by optical microscopy (scale bar, 50 μm) (c) and H&E staining (scale bar, 200 μm) (d). e Attachment of iPSC-derived EpSCs to the acellular amniotic membrane was detected by scanning electron microscopy (SEM). Cells were seeded at 1 × 105 cells/cm2. Scale bar represents 100 μm (× 200) and 25 μm (× 1000). f CCK8 analysis of iPS-EpSCs cultured on a hAAM showing a higher ability for proliferation in comparison with cells cultured on laminin

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