Fig. 4

LncRNA MBNL1-AS1 negatively regulates miR-301b-3p. a Sequence complementarity between lncRNA MBNL1-AS1 and miR-301b-3p detected by bioinformatics analysis. b miR-301b-3p expression in NSCLC tissues and adjacent normal tissues measured using RT-qPCR (n = 56). c Luciferase activity. d Binding situation between lncRNA MBNL1-AS1 and miR-301b-3p verified using RNA pull-down. e miR-301b-3p expression in A549-SP⁺ after lncRNA MBNL1-AS1 overexpression measured using RT-qPCR; *p < 0.05 vs. the adjacent normal tissues, co-transfection with miR-NC, and lncRNA MBNL1-AS1-wt. Measurement data were depicted as mean ± standard deviation. b Data was analyzed using the paired t test. c–e Comparisons between two groups were analyzed using unpaired t test. The experiment was repeated three times