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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: microRNA-301b-3p downregulation underlies a novel inhibitory role of long non-coding RNA MBNL1-AS1 in non-small cell lung cancer

Fig. 6

LncRNA MBNL1-AS1 increases TGFBR2 expression via competitive combination with miR-301b-3p. a Sequence complementarity between miR-301b-3p and TGFBR2 3′-UTR detected by bioinformatics analysis. b mRNA and protein levels of TGFBR2 in NSCLC tissues and adjacent normal tissues measured using RT-qPCR and western blot analysis (n = 56). c Luciferase activity after co-transfection. d Binding situation between miR-301b-3p and TGFBR2 verified using RNA pull-down. e TGFBR2 mRNA level in A549-SP+ measured using RT-qPCR. f TGFBR2 protein level in A549-SP+ measured using western blot analysis. NC group: cells treated with NC. miR-301b-3p-inhibitor group: cells treated with miR-301b-3p inhibition. miR-301b-3p-mimic group: cells treated with miR-301b-3p overexpression. miR-NC + oe-lncRNA MBNL1-AS1 group: cells treated with lncRNA MBNL1-AS1 overexpression and miR-NC. miR-301b-3p-mimic + oe-lncRNA MBNL1-AS1 group: cells treated with lncRNA MBNL1-AS1 overexpression and miR-301b-3p overexpression; *p < 0.05 vs. the adjacent normal tissues, co-transfection with miR-NC and TGFBR2-wt, A549 cells treated with Bio-NC, or A549 cells treated with NC. Measurement data were depicted as mean ± standard deviation. b Data was analyzed using the paired t test. c, d Comparisons between two groups were analyzed using unpaired t test. e Comparisons among multiple groups analyzed using one-way ANOVA with Tukey post hoc test used. The experiment was repeated three times. Bio, biotin

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