Fig. 5

FN precoating wound bed promotes the adhesion and proliferation of ESCs. a–c Represent area and analysis of wound tissue sections stained with K15 and Ki-67 on post-injury day 7 showing the number of ESCs and proliferating cells in rats’ dorsal wound treated with PBS (control), EBCs, and FN + EBCs. Arrows indicate the positive cells. d Analysis of wound tissue sections double-stained with K15 and Ki-67 on post-injury day 7 showing the number of growing ESCs in rats’ dorsal wound treated with PBS (control), EBCs, and FN + EBCs. e Representative qRT-PCR analysis showing relative mRNA levels of Ki-67 and K15 for each group on day 7. The values were analyzed by Graph Prism 7.0. Error bars represent SEM. Student’s t test, *P < 0.05 compared with control value (n = 6), #P < 0.05 compared with EBCs value (n = 6)