Table 1 Primer sequences for quantitative real-time reverse transcription PCR
Gene | Direction | Sequence 5′ → 3′ |
|---|---|---|
Tumor necrosis factor-α (TNF-α) | Forward | CATCCGTTCTCTACCCAGCC |
Reverse | AATTCTGAGCCCGGAGTTGG | |
Interleukin-8 (IL-8) | Forward | TCCACTCCCAGCATCGTAGA |
Reverse | CAGAGTAAAGGGCGGGTCAG | |
Interleukin-10 (IL-10) | Forward | CTTCGGCCCAGTGAAGAGTT |
Reverse | TGGAGCTTGCTAAAGGCACT | |
MKI 67 (Ki-67) | Forward | GGAGGGTTTCCAGACACCAG |
Reverse | ACTTGCCCTAGACTGTCTCTCT | |
Keratin 15 (K15) | Forward | GCTTGCTAGGGACCCGAAG |
Reverse | CTCGGGTAGAGCCACTTCCA | |
Cluster of differentiation 31 (CD31) | Forward | CTCCATCCTGTCGGGTAACG |
Reverse | TTCTTCGTGGAAGGGTCTGC | |
Matrix metallopeptidase 9 (MMP-9) | Forward | CGCCAACTATGACCAGGATA |
Reverse | GTTGCCCCCCCAGTTACAGT | |
Collagen I (Col I) | Forward | GTACATCAGCCCAAACCCCA |
Reverse | CAGGATCGGAACCTTCGCTT | |
Collagen III (Col III) | Forward | ATATGTGTCTGCGACTCGGG |
Reverse | GGGCAGTCTAGTGGCTCATC | |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Forward | AGACAGCCGCATCTTCTTGT |
Reverse | TGATGGCAACAATGTCCACT |