Fig. 3
From: Oxidative stress mediates depot-specific functional differences of human adipose-derived stem cells
Ascorbic acid treatment improves ROS and adipogenic capacity of VS-ASCs. a Graph showing ROS levels of SC- and VS-ASCs each in triplicates from S17 when treated with ascorbic acid (AA—50 μM) for 48 h and then stained with CellROX™ Deep Red Reagent. Statistical significance was calculated by ANOVA. **p < 0.01. Similar results were obtained with another subject S14. b Representative images (× 40) showing fluorescence staining of ROS in S17 SC- and VS-ASCs when treated with AA for 48 h and then stained with CellROX™. Scale bar represents 100 μm. c Graphs showing fold expression of oxidative stress-related genes XDH, NOX1, and GPx3 each in triplicates in SC- and VS-ASCs with or without AA from S17. Statistical significance was calculated by ANOVA. *p < 0.05 and **p < 0.01 when compared to SC-C; ^p < 0.05 and ^^p < 0.01 when compared to VS-C. d Graph showing GPx activity as measured by enzyme assay in SC- and VS-ASCs treated with AA each in triplicates from S17. Statistical significance was calculated by ANOVA. ***p < 0.001 when compared to SC C; ^^p < 0.01 when compared to VS C. e Graph showing relative AdipoRed staining levels of lipid droplets each in triplicates in S17 SC- and VS-ASCs that were treated with and without AA for the first 6 days (D0–6) during adipogenic stimulation. Statistical significance was calculated by ANOVA. ***p < 0.001 when compared to SC-C. ^p < 0.05 when compared to VS-C. f Representative images (× 10) showing AdipoRed staining of lipid droplets in S17 SC- and VS-ASCs with and without AA treatment. Scale bar represents 100 μm