Fig. 3

Hydrogen treatment attenuated the stemness of glioma cells. The expression levels of GFAP and CD133 in glioma cells were assessed with immunofluorescence staining after hydrogen treatment (a). The CD133-positive cell number was determined using flow cytometry analysis after hydrogen treatment (b). The cancer stem cell self-renewal capacity of glioma cells was determined by using sphere formation assays. The representative images were captured (c) and quantified (d). **p < 0.01 compared with CTRL group. GFAP, glial fibrillary acidic protein; CTRL, control; HRM, hydrogen-rich medium