Fig. 2

R-spondin2 enhances hematopoietic differentiation of hPSCs independently of culture conditions and cell lines. a Representative immunofluorescence images of BC1 cells with or without treatment of R-spondin2 (20 ng/mL) showing the generation of CD43⁺ HPCs at day 7 of mAGM-S3 co-culture. b Flow cytometry analysis of BC1 cells with or without treatment of R-spondin2 (20 ng/mL) showing the generation of CD43⁺ HPCs at day7 of mAGM-S3 co-culture. Results are shown as means ± SD (n = 3). *P < 0.05. c Representative immunofluorescence images of H9 cells with or without treatment of R-spondin2 (20 ng/mL) showing the generation of CD43⁺ HPCs at day7 of mAGM-S3 co-culture. d Flow cytometry analysis of H9 cells with or without treatment of R-spondin2 (20 ng/mL) showing the generation of CD43⁺ HPCs at day7 of mAGM-S3 co-culture. Results are shown as means ± SD (n = 3). **P < 0.01. e Representative immunofluorescence images of Z-15 cells with or without treatment of R-spondin2 (20 ng/mL) showing the generation of CD43⁺ HPCs at day7 of mAGM-S3 co-culture. f Flow cytometry analysis of Z-15 cells with or without treatment of R-spondin2 (20 ng/mL) showing the generation of CD43⁺ HPCs at day7 of mAGM-S3 co-culture. Results are shown as means ± SD (n = 3). **P < 0.01. g Representative immunofluorescence images of H1 cells with or without treatment of R-spondin2 (20 ng/mL) showing the generation of CD43⁺ HPCs at day 7 of chemical defined hematopoietic differentiation. h Flow cytometry analysis of H1 cells with or without treatment of R-spondin2 (20 ng/mL) showing the generation of CD43⁺ HPCs at day7 of chemical defined hematopoietic differentiation. Results are shown as means ± SD (n = 3). **P < 0.01. i Representative immunofluorescence images of BC1 cells with or without treatment of R-spondin2 (20 ng/mL) showing the generation of CD43⁺ HPCs at day 7 of chemically defined hematopoietic differentiation condition. j Flow cytometry analysis of BC1 cells with or without treatment of R-spondin2 (20 ng/mL) showing the generation of CD43⁺ HPCs at day 7 of chemically defined hematopoietic differentiation condition. Results are shown as means ± SD (n = 3). **P < 0.01