Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Genome-wide DNA methylation and hydroxymethylation analysis reveal human menstrual blood-derived stem cells inhibit hepatocellular carcinoma growth through oncogenic pathway suppression via regulating 5-hmC in enhancer elements

Fig. 2

MenSCs had an anticancer effect associated with dynamic epigenetic alterations in vitro. a 5-hmC level in HCC cells was measured using the IF method. b Percentage of 5-hmC in genome was detected using ELISA. c Expression of DNA methylation and demethylation enzymes in HCC cells was altered in a time-dependent manner during MenSC coculture. d Ki67 was measured using the IF method to show the proliferation state of HCC cells. e CCK8 assay was performed to determine the cell viability of HCC cells after 96 h of MenSC coculture. f Clonogenic assay was evaluated to determine the potential of clonal formation in HCC cells with MenSC coculture. Scale bar 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512