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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Genome-wide DNA methylation and hydroxymethylation analysis reveal human menstrual blood-derived stem cells inhibit hepatocellular carcinoma growth through oncogenic pathway suppression via regulating 5-hmC in enhancer elements

Fig. 5

MenSCs suppress PI3K/AKT pathway via regulating 5-hmC and 5-mC abundance at regulatory regions. a 5-hmC and 5-mC peaks in enhancers or around TSS regions in PTEN, AKT1, AKT2, and FOXO3 were significantly changed after MenSC treatment. Yellow boxes indicate the location of DHMRs and DMRs. b FPKM of transcript for key genes from RNA-Seq data. c Western blot assay indicates that protein expression of pivotal genes in the PI3K/AKT pathway after MenSCs coculture and that downstream apoptosis was activated. d Cell apoptosis and necrosis of HCC cells after MenSC treatment were clearly increased by flow cytometry with FITC-Annexin V/PI double staining. *P < 0.05, **P < 0.01, ***P < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512