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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Genome-wide DNA methylation and hydroxymethylation analysis reveal human menstrual blood-derived stem cells inhibit hepatocellular carcinoma growth through oncogenic pathway suppression via regulating 5-hmC in enhancer elements

Fig. 6

MenSCs inhibit EMT of HCC through suppressing MAPK pathway at 5-hmC and 5-mC regulatory regions. a 5-hmC levels in the enhancer and promoter regions of RAF1, MAPK1, and MAPK3 were clearly downregulated. The 5-mC level of an enhancer in MAPK1 was increased, while those in RAF1 and MAPK3 showed no significant increase. b FPKM of transcript for MAPK pathway genes correspond to the epigenetic alterations. c Protein expression of key genes in the MAPK pathway after MenSC treatment. Downstream EMT was suppressed by MenSC coculture. d, e Cell migration and invasion of HCC cells measured by wound healing assay and transwell assay after MenSC coculture. Scale bar 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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Stem Cell Research & Therapy

ISSN: 1757-6512