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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Fluorescence-based tracing of transplanted intestinal epithelial cells using confocal laser endomicroscopy

Fig. 1

Staining of human colonic organoids. Fluorescent imaging of intestinal organoids stained with fluorescent dyes or nanoparticles, along with assessments of staining quality (homogeneous, heterogeneous or unstained). Standard fluorescent microscopy of organoids stained with a fluorescein, depicting accumulation in the organoid lumen, b an inert membrane permeable dye, c CMFDA or d a carbocyanine-based cytoplasmic membrane dye. e Confocal fluorescent imaging showing membrane-associated PLGA nanoparticles that have not been internalized into the cells. Nuclei are stained with Hoechst 33342. f Standard fluorescent imaging of intestinal organoids after attempted staining with quantum dots, depicting particle aggregation in proximity of the organoid. White scale bar, 100 μm

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