Fig. 5

Imaging in vitro and ex vivo with fluorescent microscopy and CLE. a Intestinal organoids labeled with CMFDA (15 μM) and imaged in vitro on day 0 and day 3 with endoscope-based CLE. Standard gain settings were used. Approximately × 1000 magnification. No scale bar available. Image diameter approximately 100–200 μm. b Rag2−/− mice were treated with dextran sodium sulfate (DSS) for 5 days. DSS was administered for 5 days, and 4 days later (on day 9), colonic organoids derived from mT/mG mice were labeled with CMFDA and transplanted (by colonic infusion) into the Rag2−/− mice. The recipient mice were sacrificed the subsequent day (day 10), and the colonic tissue was harvested for analysis. c Brightfield and fluorescent images of colonic tissue mucosa from two different recipient mice (1 and 2) and three separate colonic regions depicting retained mT/mG (red) and CMFDA (green) positive cells. White scale bar, 1 mm. d Fluorescent images of the corresponding colonic regions (recipient mouse 1 and 2) taken with an endoscope-based CLE. No scale bar available