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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: The potential application of concentrated growth factor in pulp regeneration: an in vitro and in vivo study

Fig. 7

The effects of CGF on alizarin red staining and differentiation-related genes expression in LPS-stimulated hDPSCs. a Alizarin red staining of the hDPSCs after culture in 1× CGF with or without 1 μg/mL LPS treated for 21 days and 28 days. Representative photographs of calcified nodules stained with alizarin red S are shown. b Relative gene expression levels of DSPP, DMP-1, OPN, Runx2, and OCN of the hDPSCs after culture in 1× CGF with or without 1 μg/mL LPS treated for 21 days and 28 days (*P < .05, **P < .01). Control, normal medium with 10% serum; OM, osteogenic-induced medium group; LPS+OM, 1 μg/mL LPS with the osteogenic-induced medium group; CGF+OM, 1× CGF-conditioned with the osteogenic-induced medium group; CGF+LPS, 1× CGF and 1 μg/mL LPS with the osteogenic-induced medium group

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