Fig. 2
From: Hsa-miR-335 regulates cardiac mesoderm and progenitor cell differentiation
MiR-335 expression alteration during cardiac differentiation. a Schematic presentation of different stages of miRNA screening. b miR-335-3p (top) and miR-335-5p (bottom) expression pattern during hESC cardiac differentiation. c Gain- and loss-of-function strategy in which miR-335-specific mimics and siRNAs were transfected on day 0 of differentiation process. d Flow cytometry results showed that hESCs were efficiently transfected by miR-335 specific mimics or siRNAs along with 50 nM BLOCK-iT Alexa Fluor Red Fluorescent oligonucleotide. e The RT-qPCR confirmed significant (P value < 0.05) overexpression of miR-335-3p (top-left) and miR-335-5p (bottom-left) but significant downregulation of miR-335-3p (top- right) and miR-335-5p (bottom-right), 24 and 48 h after transfection. RT-qPCR data are presented as mean ± SEM normalized against day 0 data. GAPDH was used as a housekeeping gene