Fig. 5
From: Hsa-miR-335 regulates cardiac mesoderm and progenitor cell differentiation
MiR-335 activates TGFβ signaling pathway through targeting SMAD7. a Alterations in the expression of SMAD7 followed by overexpression and downregulation of miR-335-3p (top) and miR-335-5p (bottom). b Dual luciferase assay study confirmed a direct interaction between miR-335-3p (top) and miR-335-5p (bottom), and SMAD7 3′UTR. Alteration in the expression of TGFβR-I was assessed by RT-qPCR followed by miR-335-3p and miR-335-5p overexpression (c) and downregulation (d) in hESC. RT-qPCR data are presented as mean ± SEM normalized against mimic-scr and siRNA-scr. GAPDH was used as a housekeeping gene