Fig. 5

Analyses of mouse testes after xenotransplantation of cSSCs. a Histological analysis of the mouse testis at 10 weeks (70 days) after xenotransplantation. a–ai In mice that received cSSCs treated with FSH in vitro, GFP-positive cells were observed in the seminiferous tubules (arrow). aii In the testes of mice that received control cSSCs (not treated with FSH), GFP-positive cSSCs were not observed in the seminiferous tubules (scale bar = 100 m). b Graphic showing the number of GFP-positive cSSCs in the seminiferous tubules 10 weeks after transplantation in the control and treated groups. The second graphic shows the percentage of seminiferous tubules exhibiting GFP-positive cSSCs compared with those of the control and treated groups. **Significant difference. Unpaired t test (p ≤ 0.05). c Polymerase chain reaction (PCR) analysis of mouse testes at 10 weeks after the transplantation of cSSCs. Evaluation of the presence of dog DNA in mouse testes. Positive control (canine fibroblasts), negative control from cells (mouse fibroblasts), and the blank control