Fig. 2

Activation and inhibition of NF-κB signaling pathway in the DESCs using TNFα and BMS-345541. a, b The effects of TNFα and BMS-345541 with five different concentrations on the proliferation of DESCs were analyzed by CCK8 assay. c The expression of p65 and p-p65 associated with canonical NF-κB signaling pathway were increased after treating with TNFα at different time points. d Western blot analyses revealed that the expression of p65 and p-p65 were downregulated with the treating of BMS-345541 at different time points. e Gray level analysis of protein expression of p65 and p-p65 in TNFα-treated DESCs. f Gray level analysis of protein expression of p65 and p-p65 in BMS-345541-treated DESCs. All values were presented as the means ± SD of triplicate experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 (treatment group vs. control group)