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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Effect of canonical NF-κB signaling pathway on the differentiation of rat dental epithelial stem cells

Fig. 3

Amelogenesis-related protein and gene expressions were analyzed by Western blot and qRT-PCR tests in NF-κB-activated and NF-κB-inhibited DESCs. a Western blot analyses for the expressions of AMBN, MMP20, and KLK4 in different groups at day 3. GAPDH served as an internal control. b Gray level analysis of amelogenesis-related protein at day 3. c The gene expression of AMBN, MMP20, and KLK4 was measured by qRT-PCR test at day 3. d Western blot analyses for the expressions of amelogenesis-related protein in each group at day 7. GAPDH served as an internal control. e Gray level analysis of amelogenesis-related protein at day 7. f qRT-PCR analysis for amelogenesis-related genes in different groups at day 7. All values were presented as the means ± SD of triplicate experiments. *P < 0.05, **P < 0.01, and ***P < 0.001

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