Fig. 6

The cross-talk between NF-κB and canonical TGF-β signaling mediated by SMAD7 during the regulation of DESC differentiation. a Expression change of SMAD7 was estimated using qRT-PCR test. b DESCs were transfected with SMAD7-targeting and control non-targeting siRNA duplexes as indicated. After 2 h, cells were treated with TNFα (10 ng/ml) for 3 days. Expressions of amelogenesis-related proteins and canonical TGF-β signaling components were assessed by Western blot analysis. c Gray level analysis of proteins in b. All values were presented as the means ± SD of triplicate experiments. *P < 0.05, **P < 0.01, and ***P < 0.001