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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Examining the fundamental biology of a novel population of directly reprogrammed human neural precursor cells

Fig. 1

Generation and in vitro characterization of drNPCs. a Schematic of protocol for reprogramming cells to drNPCs. b At passage 7, drNPCs continue expressing high levels of Nestin, Sox2, GFAP, NG2, Ascl1, Tuj1, Dcx, and Pax6. Nuclei stained with Hoechst (blue). Scale bar = 100 μm. c drNPCs at passage 12 were analyzed for expression of multiple markers, graphed by intensity of expression to determine cell population purity, using a 12-channel Amnis FlowSight® Imaging flow cytometer. d Normal karyotype of drNPCs at passage 12

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