Fig. 6

drNPC transplants in myelin-deficient Shiverer mice. drNPCs give rise to MBP and GFAP expressing cells post-transplant (A, B). STEM121 (red) expressing drNPCs express MBP (A_iii; green) and GFAP (B_iii; turquoise) in the CC (outlined with dotted lines). C, D MBP and GFAP expression in the transplanted drNPCs between 1 week and 2 weeks post-transplant. E, F A subpopulation of transplanted drNPCs are HuNu⁺/Ki67⁺ on the corpus callosum of Shi^−/− mice at 1 week (A) and 2 weeks (B) post-transplant. White arrows indicate Ki67⁺/HuNu⁺ cells. There is no significant difference between the total number of HuNu⁺ drNPCs at the site of the transplant between 1 and 2 weeks post-transplant (G). There is a significant decrease in the percentage of Ki67⁺/HuNu⁺ drNPCs between 1 and 2 weeks post-transplant (H). Dotted lines indicate the border of corpus callosum border. Cx cortex, CC corpus callosum, LV lateral ventricle; scale bars = 50 μm, n = 4 transplanted hemispheres per group, * = p < 0.05; n.s. not significant