Fig. 2

Hypoxic stress increased autophagy in BM-MSCs. a Representative Western blots of LC3-I/LC3-II, Beclin-1, and P62 in BM-MSCs subjected to normal and hypoxic conditions. Quantification of the protein expression levels of LC3-II/ LC3-I (b), Beclin-1 (c), and p62 (d) at the indicated time points. e Representative immunofluorescence images of red fluorescent protein (RFP)-green fluorescent protein (GFP)-LC3 (red fluorescence) in BM-MSCs under normal conditions and H/SD (scale bars, 10 mm). f Quantification of autophagy was presented as the numbers of puncta in LC-3 positive cells (n = 5, p < 0.05). Data are expressed as the means ± SEM; n = 5; *p < 0.05