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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Mesenchymal stem cells overexpressing hepatocyte nuclear factor-4 alpha alleviate liver injury by modulating anti-inflammatory functions in mice

Fig. 2

HNF-4α did not affect MSC homing. The migration and proliferation of MSCs were tested in vivo and in vitro. a Recruitment of MSCs was tested on the third day and seventh day after injection by fluorescent analysis (× 200). b Three fields of view were selected for each group to count the EGFP-positive cells. The mean of the three fields was used to represent the migrated cells for each group. c Transwell assays were performed to verify the migration of MSCs in vitro (× 200). Cell numbers were counted after 72 h. d Quantification of panel c. e A CCK-8 assay was performed to detect the proliferation of MSCs at 48 h. (Five mice were used in each group. The data are represented as the mean ± S.D.) Scale bars, 100 μm

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