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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Mesenchymal stem cells overexpressing hepatocyte nuclear factor-4 alpha alleviate liver injury by modulating anti-inflammatory functions in mice

Fig. 4

HNF-4α-MSCs exerted enhanced anti-inflammatory effects by increasing iNOS expression. a MSCs were treated with 10 ng/mL IFN-γ and 10 ng/mL TNF-α for 12 h and then co-cultured with A1.1 cells. After 24 h, the proliferation of A1.1 cells was detected using a 3H-Tdr assay. b, c MSCs were treated with 10 ng/mL IFN-γ and 10 ng/mL TNF-α for 10 h and 24 h. The expression of iNOS was detected using western blotting and RT-PCR assays. GAPDH was used as an internal control. d Conditioned medium from MSCs treated with 10 ng/mL IFN-γ and 10 ng/mL TNF-α was collected after 24 h. I+T, IFN-γ and TNF-α. Nitrate levels were detected (The data are represented as the mean ± S.D.)

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