Fig. 5

HNF-4α promoted the immune regulation of MSCs through NF-κB signalling. a P65 expression was detected in MSCs after TNF-α and IFN-γ treatment for 2 h by immunofluorescence staining. Nuclei were stained with DAPI (× 200). b The percentage of cells containing P65 in the nucleus was calculated. c Expression of IκB-α was detected by western blotting in MSCs after 10 ng/mL IFN-γ and 10 ng/mL TNF-α treatment for 2 h. GAPDH was used as an internal control. d Expression levels of IκB-α (treatment for 2 h) and iNOS (treatment for 24 h) were detected after NF-κB inhibitor BAY 11-7082 treatment in HNF-4α-MSCs. GAPDH was used as an internal control. I+T, IFN-γ and TNF-α. (The data are represented as the mean ± S.D.) Scale bars, 100 μm