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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Differentiation of primordial germ cells from premature ovarian insufficiency-derived induced pluripotent stem cells

Fig. 6

The dynamics of methylation and histone modifications in the induced PGCs from POI-1-iPSCs. a The CpGs of imprinted genes (H19, PEG1, and SNRPN DMRs) were analyzed following bisulfite sequencing from POI-1-iPSCs, iPSCs-11, PGCs from POI-1-iPSCs, PGCs from iPSCs-11, human ESCs, and HEF. Percentage methylation was calculated comparing the number of CpG methylated sites in undifferentiated iPSCs before differentiation with the number of CpG methylated sites at the same locus in the PGC. b Immunofluorescence for H3K9me3 (red) exhibit 100% (13/13) prominent H3k27me3 unclear body in differentiated human embryonic fibroblasts (EF), c 56.25% (9/16) prominent H3k27me3 unclear body in the induced PGCs from POI-1-iPSCs. While undifferentiated POI-1-iPSCs (d) and iPSCs-11 cells (f) showed diffuse staining

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