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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Effect of ectopic high expression of transcription factor OCT4 on the “stemness” characteristics of human bone marrow-derived mesenchymal stromal cells

Fig. 3

Identification of OCT4 expression in ALL MSCs. a The expression of OCT4 protein was analyzed with FCM at various subcloning time. The green curves indicated the isotype antibody control. Control MSCs refer to the untransfected parental ALL MSCs. Negative control refers to the antibody replaced by PBS. b The expression of OCT4 was determined by CIFA in transfected MSCs (the upper panel refers to the untransfected parental ALL MSCs, while the lower panel refers to stably transfected MSCs) (the nuclei are stained with DAPI). c. OCT4 mRNA was analyzed by RT-PCR at various subcloning times to verify the FCM data. OCT4-1143bp indicates the full length of OCT4 ORF sequence, and OCT4-247bp represents the specific length of OCT4. GAPDH served as an internal control. RT(-) refers to the template replaced by water

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Stem Cell Research & Therapy

ISSN: 1757-6512