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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Effect of ectopic high expression of transcription factor OCT4 on the “stemness” characteristics of human bone marrow-derived mesenchymal stromal cells

Fig. 4

Effect of OCT4 overexpression on the expression of stemness-related transcription factors (TFs) by FCM and RT-PCR. A Comparison of the expression levels of stemness-related TFs (NANOG, SOX2, OCT4) by FCM between the OCT4 overexpression and the parental untransfected MSCs. B The expression levels of stemness-related TFs detected by FCM at different subcloning time points in OCT4 overexpression and untransfected MSCs. The results represented mean ± SD. C mRNA of the three TFs were analyzed by RT-PCR to verify the FCM data. GAPDH serves as an internal control. RT(-) refers to the template replaced by water. Lanes 2–4 refer to the three different ALL samples with OCT4 stable overexpression. D The morphology changes of ALL MSCs after transfection with OCT4 (b) and after the third time subcloning (c), and (a) refers to the untransfected P4 MSCs (× 10)

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Stem Cell Research & Therapy

ISSN: 1757-6512