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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Effect of ectopic high expression of transcription factor OCT4 on the “stemness” characteristics of human bone marrow-derived mesenchymal stromal cells

Fig. 5

Evaluation the effect of OCT4 overexpression on the expression of ESC-related surface markers and ESC-like properties by FCM, CIFA, and RT-PCR. A The expressions of ESC surface markers (SSEA4, TRA-1-60, and TRA-1-81) in OCT4 overexpression ALL MSCs were examined by CIFA. B The morphology changes of ALL MSCs with OCT4 stable overexpression in differentiation medium (a: the untransfected MSCs as the control; b: the EBs of MSCs-OCT4; c, d: EBs at different differentiation time points (c: day 3, d: day 7). C The expression levels of ESC-related surface markers were verified with FCM in control untransfected (upper panel) and OCT4 overexpression MSCs (lower panel). D The expression of ecto-, endo-, and mesoderm genes analyzed by RT-PCR at various differentiation time points (day 7 and day 14) in untransfected and OCT4 overexpression MSCs (“U” refers to undifferentiation and “D” refers to differentiation) (ectoderm gene: TUBB3, WNT1; endoderm gene: SPARC; mesoderm gene: TBX20, Brychury)

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Stem Cell Research & Therapy

ISSN: 1757-6512