Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Elevated level of mitochondrial reactive oxygen species via fatty acid β-oxidation in cancer stem cells promotes cancer metastasis by inducing epithelial–mesenchymal transition

Fig. 4

Mitochondrial ROS elevated significantly in RH-TS. a After isolation of RH-TS and RL-TS cells from 4 T1 tumor spheres, the ROS composition was measured by DCFH-DA, DAF-FM, DHE, and MitoSox Red as described in Materials and Methods. Scale bar = 50 μm. *** p < 0.001. b The ROS composition of RH-TS and RL-TS cells in HCT116, SW480, and HT29 cells was measured by DCFH-DA, DHE and MitoSOX Red. Results are shown as mean ± SEM from three independent experiments. Scale bar = 50 μm. *** p < 0.001. c RH-TS cells increased MitoSox Red level significantly. MitoSox Red levels of parental cells (blue), RL-TS (green), RH-TS (red) were analyzed by flow cytometry. Three independent experiments performed

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512