Fig. 6

MSCs-E1s display tumor tropism and good intratumoral dissemination. a MSCs-E1s displayed promising tumor tropism. Nude mice bearing subcutaneous Ki-ras prostate tumors received CMTMR-labeled human MSCs-E1s (intracardiac administration). Mice were euthanized at 24, 48, and 72 h post-injection; tumors and tissues were excised, homogenized in RIPA buffer on ice, and fluorescence was measured. Bars indicate % of total recovery of fluorescence. b MSCs-E1s display good intratumoral dissemination. Intravital microscopy (× 4) showing TrampC2 tumor spheroids (Green) growing in dorsal skinfold chambers in nude mice. 3 × 10⁶ CMTMR-labeled MSCs-E1s (red) given via intracardiac administration. Observations were made at 1 h (top row), 24 h (second row), 48 h (third row), and 72 h (fourth row). The first column shows light images of chambers. Middle column shows green fluorescence of tumor. The last column shows red fluorescence of MSCs-E1s that are well disseminated within tumor masses