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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: The long non-coding RNA Snhg3 is essential for mouse embryonic stem cell self-renewal and pluripotency

Fig. 4

Snhg3 overexpression promotes mouse embryonic stem cell (mESC) self-renewal and inhibits differentiation. a Stable Snhg3-overexpressing mESCs were generated using a lentivirus. GFP-mESCs were generated as a control. qRT-PCR was used to access Snhg3 levels. Secondary colony formation assays were performed by re-plating different cell numbers (200, 400, or 800 cells per well) onto feeders for 7 days (b). Total colony numbers were quantified after alkaline phosphatase (AP) staining (c). qRT-PCR analysis of the expression of pluripotency markers (d) or differentiation markers (e) in Snhg3- or GFP-overexpressing mESCs. f Embryoid bodies (EBs) formed by GFP- or Snhg3-overexpressing mESCs were visualized by phase contrast microscopy after day 3 or 6; × 4 objective. Data are presented as the mean ± SD; n = 3, two-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001 for all panels

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