Fig. 5

Snhg3 is essential for mouse embryonic development. a Morphology of E3.5 mouse embryos after siControl or siSnhg3 treatment; × 10 objective. b siControl- or siSnhg3-injected zygotes were cultured in vitro until E4.5 and the blastocyst/non-blastocyst rate was quantified. siControl, n = 50; siSnhg3, n = 64. ***p < 0.001, chi-square test. c qRT-PCR analysis of Snhg3, Nanog, and Oct4 expression in blastocysts after siControl or siSnhg3 treatment. Data are presented as the mean ± SD; n = 3, two-way ANOVA. **p < 0.01, ***p < 0.001. d Immunostaining of Nanog (green) and Oct4 (red) in blastocysts after siControl or siSnhg3 treatment. The nuclei were stained with DAPI (blue); × 20 objective. e The percentage of Nanog- or Oct4-positive cells in blastocysts was quantified based on the staining in d. n, number of embryos analyzed. *p < 0.05, two-way ANOVA