Fig. 1

Characterization of the human embryonic stem cell reporter line ESC-Rep. a Identifying the DNA sequences of the three reporter genes GFP, HSVtk, and Fluc in ESC-Rep by semiquantitative PCR. GAPDH was used as a reference. b Immunofluorescence staining of ESC-Rep with pluripotency markers (NANOG and POU5F1). Nucleus is marked by Hoechst 33342 (blue). Scale bar, 50 μm. c Real-time PCR analysis of genes associated with pluripotency in ESCs and ESC-Rep cells. d Real-time PCR analysis of pluripotency markers (POU5F1 and NANOG) in ESC-Rep and differentiating EBs produced from ESC-Rep (ESC-Rep-EBs). e Real-time PCR analysis of germ layer markers in undifferentiated ESC-Rep and ESC-Rep differentiated EBs (ESC-Rep-EBs) (MESP1 and EVX1 for mesoderm, SOX1 and PAX6 for ectoderm, FOXA2 and GATA6 for endoderm). All data are presented as the mean ± SEM; Student’s t test; *p < 0.05, and ns, not significant