Fig. 3

ESC-Rep-CM injection in two heart ischemia models. a Representative echocardiogram of mice at day 28 in the Sham control, PI group after PBS or ESC-Rep-CM injection, and the IR group after the injection of PBS or ESC-Rep-CMs. n = 6 per group. b Quantitative analysis of left ventricular ejection fraction (LVEF) in PBS or ESC-Rep-CM-injected PI mice from day − 1 (baseline) to day 28. c Quantitative analysis of left ventricular fractional shortening (LVFS) in PBS or ESC-Rep-CM-injected PI mice from day − 1 to day 28. d Quantitative analysis of LVEF in the IR groups from day − 1 to day 28. e Quantitative analysis of LVFS in the IR groups from day − 1 to day 28. f Representative Masson trichrome staining images of PI and IR hearts at day 28 after PBS or ESC-Rep-CM injection. Scale bar, 1 mm. n = 3 per group. g Statistical analysis of relative fibrosis area from Masson trichrome staining data. n = 3 per group. h Real-time PCR analysis of heart tissue for fibrosis markers in the Sham control, PI group treated with PBS or ESC-Rep-CMs, and IR groups treated with PBS or ESC-Rep-CMs at day 28. n = 3 per group. All data are presented as the mean ± SEM; one-way ANOVA or two-way repeated-measures ANOVA; * p < 0.05, and ns, not significant