Fig. 4

Immunohistochemical staining of α-SMA for smooth muscle regeneration after transplantation of collagen/transfected En-PSCs. Immunohistochemical staining of α-SMA for smooth muscle regeneration at 30 days (A–E) and 90 days (F–J) in sham group (A, F), collagen/PBS group (B, G), collagen/En-PSCs group (C, H), collagen/si-CYR61 En-PSCs group (D, I), and collagen/ov-CYR61 En-PSCs (E, J). Arrowheads indicate repair sites. Scale bar, 500 μm. Local magnification of figures (A–J) were showed in the pictures (A′–J′). Scale bar, 200 μm. K The statistical analysis of the ratio of positive area in the regenerative uterine horn to the total area of the uteri. Each experiment was repeated four times. Data were presented as mean ± SEM. *P < 0.05 and **P < 0.01