Fig. 1

Healthy donor-derived human-induced pluripotent stem cell (hiPSC) generation from non-mobilized peripheral blood (PB)-derived CD34⁺ hematopoietic stem and progenitor cells (HSPCs) using SeVdp(KOSM)-302L. a Schematic diagram illustrating the schedule of hiPSC generation. b Percentages of cells expressing CD34, as assessed by FACS analysis of non-enriched peripheral blood mononuclear cells (PBMCs), a flow-through population (flow-through), and the CD34⁺-selected cells (CD34⁺ cells). The results demonstrate significant enrichment of the CD34⁺ cells (up to 60.8%) after immunomagnetic bead selection. c Sequential images of a representative colony derived from SeVdp(KOSM)-302L-transduced CD34⁺ cells, showing a phase of initial proliferation (day 1–day 4), followed by the formation of spherical colony-like structures (day 5–day 11). Also shown are images of typical hiPSC-like colonies that appeared during the subsequent expansion phase (day 17 and day 37). Magnified images are shown in insets for clarity. P1 and P4 indicate passage 1 and passage 4, respectively. d Colony formation efficiency of each seeded cell type. PBMCs, flow-through cells, and CD34⁺ cells were tested after infection with SeVdp(KOSM)-302L. Each bar represents the efficiency assessed in each individual well. The mean efficiency values for PBMCs (0.17%), flow-through (0%), and CD34⁺ PBMCs (5.58%) are shown. e Fluorescent images of live colonies stained with anti-TRA-1-60 antibody (red, top), anti-TRA-1-81 antibody (green, middle), or the CDy1 dye (red, bottom). Nuclei were stained with Hoechst 33342 dye (blue)