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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34+ cells using the auto-erasable Sendai virus vector

Fig. 2

Establishment of a panel of patient-derived human induced pluripotent stem cell (hiPSC) lines from non-mobilized peripheral blood-derived CD34+ hematopoietic stem and progenitor cells (HSPCs) using SeVdp(KOSM)-302L. a Sequential images of representative primary colony growth observed in the process of TkSLE5 (top) and TkSCR1 cell line establishment (bottom). b Live cell image of a CDy1 dye-stained (red) representative primary colony obtained at day 13 in the course of TkSPR2 cell line generation. c Scatter plots indicating the colony formation efficiency of the seeded cells (y-axis) and the estimated number of hiPSC colonies obtained from 1 mL of blood (x-axis) from 15 patients. d Table showing the failure rates in the colony pickup step (top) and in the subsequent hiPSC expansion process (bottom). e Bar chart showing the step-by-step progress in establishing hiPSC lines from the healthy donor (TkPP2) and 15 patients. For clarity, bars are uniquely colored for each passage number: P0 (passage 0) in blue, P1 (passage 1) in green, P2 (passage 2) in orange, and P3 (passage 3) in yellow. The gray line shows a typical time point (day 11) for colony pickup, but the actual pickup dates varied between day 8 and day 14. f Images of the healthy donor (TkPP2)- and 15 patient-derived hiPSC lines cultured under feeder-free conditions after establishment, showing a typical monolayered colony appearance

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