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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34+ cells using the auto-erasable Sendai virus vector

Fig. 4

Analysis of the T cell receptor gamma (TCRγ) gene locus rearrangement. a Electrophoretic images showing the results of detection analysis for the specific TCRγ gene rearrangement, conducted using the T Cell Receptor Gamma Gene Rearrangement Assay kit for Gel Detection. Top: analysis of 9 independent TkPP2 lines. Bottom: analysis of 9 patient-derived human induced pluripotent stem cell (hiPSC) lines (names are indicated). NTC, non-template control. IVS-0009 and IVS-0000 are the monoclonal and polyclonal samples, respectively, provided as positive controls in the kit. Another positive control used was TkSST2-2 (top panel), a monoclonal T cell-derived hiPSC line established previously in our laboratory. Note that there are no single detectable bands in the test sample lanes. b Summary of the results of the TCRγ gene rearrangement analysis. No positive results were seen in the tested samples (38 hiPSC lines in total)

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Stem Cell Research & Therapy

ISSN: 1757-6512