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Table 2 Amounts of PB, PBMCs, and CD34+-enriched cells used for reprogramming

From: Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34+ cells using the auto-erasable Sendai virus vector

Healthy donor and patients (hiPSC line) Amounts of PB and cryopreserved PBMCs used for hiPSC generation (% of live PBMCs after thawing) No. of CD34+-enriched cells for transfection
Healthy (TkPP2) 1.0 × 107 cells 15,000
SLE (TkSLE3) 8.8 mL 0.4 × 107 cells (33%) 13,000
SLE (TkSLE4) 18.4 mL 1.0 × 107 cells (50%) 16,000
SLE (TkSLE5) 7.5 mL 1.0 × 107 cells (71%) 39,375
PM (TkSPD3) 7.5 mL 0.5 × 107 cells (52%) 11,500
PM (TkSPD4) 4.2 mL 1.0 × 107 cells (61%) 12,5000
PM (TkSPD5) 8.8 mL 1.0 × 107 cells (38%) 14,000
X-CGD (TkSCG3) 13.1 mL 1.0 × 107 cells (53%) 24,700
X-CGD (TkSCG4) 8.5 mL 1.0 × 107 cells (56%) 9000
PID (TkSPR1) 5.3 mL 1.0 × 107 cells (76%) 18,000
PID (TkSPR2) 7.7 mL 1.0 × 107 cells (38%) 31,000
JIA (TkSCR1) 4.2 mL 1.0 × 107 cells (65%) 33,375
CMS (TkS42–1) 13.9 mL 1.0 × 107 cells (21%) 5500
MD (TkSmD1) 20.0 mL 1.0 × 107 cells (12%) 63,000
MD (TkSmD3) 4.7 mL 0.5 × 107 cells (56%) 55,000
KCS2 (TkSKCII2) 4.8 mL 0.5 × 107 cells (68%) 42,000
  1. The amounts of peripheral blood (PB) and peripheral blood mononuclear cells (PBMCs) used in the generation of these human induced pluripotent stem cell (hiPSC) lines are listed. The PB volume shown here was calculated from the corresponding PBMC numbers contained in each frozen vial (cryopreserved PBMCs). The number of PBMCs represents the value before cryopreservation. After thawing, a certain level of cell death occurs; % live PBMCs are thus shown in parentheses, calculated by counting the actual number of living cells recovered after thawing. The number of CD34+-enriched cells used for SeVdp(KOSM)-302L transduction is also indicated
  2. SLE systemic lupus erythematosus, PM polymyositis, X-CGD X-linked chronic granulomatous disease, PID primary immunodeficiency, JIA juvenile idiopathic arthritis, CMS congenital malformation syndrome, MD mitochondrial diabetes, KCS2 Kenny-Caffey syndrome type 2