Table 2 Adult stem cells strategies for human endothelial cell derivation
From: Strategies for derivation of endothelial lineages from human stem cells
Study | Cell line | Induction system | Purification method | Culture milieu | Induction period (days) | Efficiency | Positive control | In vitro analysis | In vivo analysis | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Medium | Growth factors | Other special factors | EC markers analyzed | Application/functional assessment | ||||||||
Zhang et al. [103] | DPSCs | In vivo differentiation system | No | No | No | No | 4 weeks | No | HDMEC | Protein analysis for cd31 | No | Blood vessel formation in mice |
Joddar et al. [83] | BMMSCs | Co-culture system (with fixed ECs/with EC matrix) | No | EBM-2 | No | No | 7 days | About 70% out of total cells | No | Protein analysis for CD31 | No | No |
Lozito et al. [84] | BMMSCs | Co-culture system (direct MSC-EC co-culture/indirect MSC-EC co-Culture/with EC matrix) | DiI-labeled FACS sorting | EGM-2-MV | VEGF | – | 10 days | No | No | Gene analysis for PECAM Protein analysis for PECAM | Tube formation on Matrigel | No |
Gong et al. [107] | SHED | Co-culture system (with EC matrix) | No | EGM-2-MV | VEGF | – | 14 days | No | No | Gene analysis for CD31, VEGFR2, VWF | Tube formation on Matrigel | No |
Oswald, J. et al. [85] | BMMSCs | Growth factor system | No | DMEM | VEGF | – | 7 days | Not clear | No | Protein analysis for KDR, VE-cad, Flt1, vWF | Tube formation on Matrigel | No |
Lloyd-Griffith et al. [115] | AFSCs | Growth factor system | No | EndoGroTM-VEGF complete media | VEGF hFGF EGF et al. | – | 14 days | No | HUVECs | Gene analysis for CD31, VEGFR2, VWF, Angiopoietin 1 Protein analysis for CD31; | In vitro Matrigel sprouting assay LDL uptake assay | No |
Xu et al. [116] | SHED/DPSCs | Growth factor system | No | EGM | VEGF | SB-431542 | 14 days | No | No | Gene analysis for VEGFR1, VEGFR2, CD31, Tie-2, EphrinB2 Protein analysis for CD31, VEGFR2 | In vitro Matrigel sprouting assay | Matrigel plug assay |
Yuan et al. [86] | hMSCs | Mechanical stimuli system | No | DMEM | bFGF | No | 7 days | No | No | Protein analysis for CD31, VE-cadherin, vWF | No | No |
Wu et al. [119] | PDMCs | Mechanical stimuli system | No | EGM | VEGF | No | 4 days | No | No | Gene analysis for Flt-1, Flk-1, vWF, PECAM-1 Protein analysis for vWF, PECAM-1 | LDL uptake assay Tube formation on Matrigel | No |