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Table 2 Adult stem cells strategies for human endothelial cell derivation

From: Strategies for derivation of endothelial lineages from human stem cells

Study Cell line Induction system Purification method Culture milieu Induction period (days) Efficiency Positive control In vitro analysis In vivo analysis
Medium Growth factors Other special factors EC markers analyzed Application/functional assessment
Zhang et al. [103] DPSCs In vivo differentiation system No No No No 4 weeks No HDMEC Protein analysis for cd31 No Blood vessel formation in mice
Joddar et al. [83] BMMSCs Co-culture system (with fixed ECs/with EC matrix) No EBM-2 No No 7 days About 70% out of total cells No Protein analysis for CD31 No No
Lozito et al. [84] BMMSCs Co-culture system (direct MSC-EC co-culture/indirect MSC-EC co-Culture/with EC matrix) DiI-labeled FACS sorting EGM-2-MV VEGF 10 days No No Gene analysis for PECAM Protein analysis for PECAM Tube formation on Matrigel No
Gong et al. [107] SHED Co-culture system (with EC matrix) No EGM-2-MV VEGF 14 days No No Gene analysis for CD31, VEGFR2, VWF Tube formation on Matrigel No
Oswald, J. et al. [85] BMMSCs Growth factor system No DMEM VEGF 7 days Not clear No Protein analysis for KDR, VE-cad, Flt1, vWF Tube formation on Matrigel No
Lloyd-Griffith et al. [115] AFSCs Growth factor system No EndoGroTM-VEGF complete media VEGF hFGF EGF et al. 14 days No HUVECs Gene analysis for CD31, VEGFR2, VWF, Angiopoietin 1 Protein analysis for CD31; In vitro Matrigel sprouting assay LDL uptake assay No
Xu et al. [116] SHED/DPSCs Growth factor system No EGM VEGF SB-431542 14 days No No Gene analysis for VEGFR1, VEGFR2, CD31, Tie-2, EphrinB2 Protein analysis for CD31, VEGFR2 In vitro Matrigel sprouting assay Matrigel plug assay
Yuan et al. [86] hMSCs Mechanical stimuli system No DMEM bFGF No 7 days No No Protein analysis for CD31, VE-cadherin, vWF No No
Wu et al. [119] PDMCs Mechanical stimuli system No EGM VEGF No 4 days No No Gene analysis for Flt-1, Flk-1, vWF, PECAM-1 Protein analysis for vWF, PECAM-1 LDL uptake assay Tube formation on Matrigel No