Table 2 Summary of relevant information of the cynomolgus primates before/after transplantation
From: Safety and efficacy of ex vivo expanded CD34+ stem cells in murine and primate models
Items | B-1 | B-2 | B-3 | N-1 | N-2 | N-3 | E-1 | E-2 | E-3 | E-4 | E-5 | Mediana | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Age (year) | 7 | 7 | 7 | 6 | 7 | 7 | 6 | 7 | 7 | 7 | 6 | 7 | |
Sex | Male | Male | Male | Male | Male | Male | Male | Male | Male | Male | Male | NA | |
Weight (kg) | 6.5 | 5.6 | 8.0 | 6.2 | 6.4 | 6.1 | 5.8 | 4.6 | 6.6 | 6.6 | 6.6 | 6.4 | |
Before expansion | Cell number | – | – | – | – | – | – | 3.0× 106 | 2.6× 106 | 3.6× 106 | 2.7× 106 | 2.4× 106 | 2.7× 106 |
CD34+ cell purity (%) | – | – | – | – | – | – | 76.0% | 64.9% | 61.5% | 73.6% | 72.1% | 72.1% | |
After expansion | Total cell number | – | – | – | – | – | – | 2.0 × 107 | 1.7 × 107 | 2.3 × 107 | 2.3 × 107 | 2.2 × 107 | 2.2 × 107 |
CD34+ cell purity (%) | – | – | – | – | – | – | 71.7% | 55.9% | 57.1% | 68.3% | 65.6% | 65.6% | |
Expansion fold | Total cell | – | – | – | – | – | – | 8.0 | 7.4 | 6.8 | 10.0 | 10.8 | 8.0 |
CD34+ cell | – | – | – | – | – | – | 7.5 | 6.4 | 6.3 | 9.3 | 9.8 | 7.5 | |
Transduction efficiency (GFP %) | – | – | – | – | – | – | 31.4% | 37.1% | 32.8% | 31.2% | 40.4% | 32.5% | |
Transplant dose (per kg) | CD34+ cell | – | – | – | – | – | – | 2.4 × 106 | 2.0 × 106 | 2.0 × 106 | 2.4 × 106 | 2.2 × 106 | 2.2 × 106 |
CD34− cell | – | – | – | 2.6 × 107 | 2.0 × 107 | 2.5 × 107 | 2.6 × 107 | 2.5 × 107 | 2.0 × 107 | 2.5 × 107 | 2.5 × 107 | 2.5 × 107 | |
Outcome | Alive | Alive | Died | Alive | Alive | Died | Alive | Alive | Alive | Alive | Alive | NA | |