Table 2 Result summary after expanding cells in different medium formulations associated with “activating” or “neutral” cues. Activation of cells occurred randomly regardless of the supplement used. Nonetheless, differentiation potential was not altered (please refer to Additional file 1: Figure S2)
Cell line | Passage number | supplement | %CD31 | %CD45 | %HLA-DR | %CD73 | %CD105 | %CD90 |
|---|---|---|---|---|---|---|---|---|
1 | P0 | 3.6 | 1.9 | 17.1 | 99.8 | 99.9 | 99.83 | |
P1 | Activating | 2.0 | 0.3 | 14.2 | 99.7 | 99.9 | 99.6 | |
P1 | Neutral | 7.7 | 2.0 | 29.6 | 99.3 | 99.4 | 99.4 | |
2 | P0 | 6.4 | 1.1 | 19.4 | 100 | 98.7 | 99.9 | |
P1 | Activating | 8.4 | 3.0 | 37.7 | 99.7 | 99.6 | 99.9 | |
P1 | Neutral | 7.6 | 3.3 | 40.9 | 99.8 | 99.5 | 99.9 | |
3 | P0 | 7.2 | 1.0 | 15.0 | 99.7 | 99.7 | 99.84 | |
P1 | Activating | 4.7 | 3.3 | 23.9 | 99.4 | 99.4 | 99.5 | |
P1 | Neutral | 4.3 | 2.9 | 25.4 | 99.1 | 99.3 | 99.3 |