Fig. 2

Lung cancer cells induce ADSCs to express myofibroblast markers accompanied by the secretion of IL-6 family cytokines. a H&E staining and immunohistochemical analysis demonstrate the expression of the myofibroblast marker α-SMA in epidural ADSCs. ADSCs treated with CM from one of four lung cancer cell lines for 48 h expressed high levels of α-SMA. Scale bar = 100 μm. b Western blotting analysis of α-SMA and FAP expression in epidural ADSCs treated with CM from one of four lung cancer cell lines for 48 h. ADSCs cultured in untreated medium served as controls. c qRT-PCR analysis of IL-6, IL-11, LIF and Acta2 mRNA expression in epidural ADSCs treated with CM from one of four lung cancer cell lines for 48 h. ADSCs cultured in untreated medium served as controls. d ELISA of IL-6, IL-11 and LIF in supernatants of ADSCs treated with lung cancer cell CM for 24 h. The supernatants of the four lung cancer cell lines and ADSCs cultured in untreated medium served as controls. ***P < 0.001