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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Small molecule inhibitor of TGF-β signaling enables robust osteogenesis of autologous GMSCs to successfully repair minipig severe maxillofacial bone defects

Fig. 1

Isolation and identification of human GMSCs. a Typical morphology of fibroblast-like cells growing out from a gingival tissue fragment after culturing 5 days. When the cells reached 80% confluence from the passage 0 for about 10 days, they were subcultured and proliferated. The fibroblast-like cells were digested by trypsin and had high purity with a homogeneous spindle shape at passage 2 for 2 weeks. Scale bar, 500 μm. b and c Results of flow cytometry analysis were showed of the expression of cell surface markers related to mesenchymal stem cells (CD44, CD73, CD90, and CD105), hematopoietic stem cells (CD34 and CD45), or macrophage and B-cells (CD19, CD11b, HLA-DR, and HLA-DQ). CD, cluster of differentiation. n = 3. Error bars represent the SD. d The colonies were formed by human gingival mesenchymal stem cells (hGMSCs) at low seeding density for 2 weeks culture. Scale bar, 500 μm

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