Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Small molecule inhibitor of TGF-β signaling enables robust osteogenesis of autologous GMSCs to successfully repair minipig severe maxillofacial bone defects

Fig. 4

In vivo bone regeneration of hGMSCs in immunocompromised mice subcutaneously. a Three-dimensional (3D) visualization based on the CBCT images of mice.3D image creation of soft tissue (a1), 3D rendering of materials at mouse bone density (a2), and maximum intensity projection (MIP) full range visualization (a3) were created using the Software NNT viewer. CBCT, cone beam computed tomography. b Macroscopic view of allograft subcutaneous transplanted in immunocompromised mice skin at 12 weeks. The graft Bio-Oss® at upper left (b1), Bio-Oss®/hGMSCs at lower left (b2), and Bio-Oss®/hGMSCs/SB431542 at lower right (b3). c Representative images of specimen stained with H&E and Masson staining retrieved from immunocompromised mice after implantation for 12 weeks. CT, connective tissues; NB, new bone. Scale bars, 200 μm. H&E, Hematoxylin-Eosin; hGMSCs, human gingival mesenchymal stem cells; SB,1 μM SB431542. d Quantification of the new bone formation with H&E and Masson staining. n = 4 mice/group, 5 views each. n = 4 mice/group. Data represent mean ± SEM. *p < 0.05, ***p < 0.001, one-way ANOVA with Tukey’s multiple comparison test

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512