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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Predifferentiated amniotic fluid mesenchymal stem cells enhance lung alveolar epithelium regeneration and reverse elastase-induced pulmonary emphysema

Fig. 5

Expression and localization of eGFP-labeled transplanted cells in the lung of PPE-induced pulmonary emphysema mouse model. a Quantitative real-time RT-PCR and b Western blot analysis were performed to detect GFP expression in the lung tissues of mice after 4-week period of PBS, AFMSCs, or LEPLCs treatment following PPE administration. Values were normalized to Gapdh or β-actin levels and were expressed relative to the PBS group. *p < 0.05, **p < 0.01, and ***p < 0.001. Each dot represents an individual mouse with the mean shown for n > 5 per group. c Immunofluorescence staining for the distribution of GFP in lung tissues. Green signal observed in the lung section indicates GFP-positive cells and nuclei were stained with DAPI (blue). Magnified views with 20 μm bars (third row) expand on the boxed regions in the low-magnified images with 100 μm bars (second row)

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